studying the effect of Rhodamine b concentration on the spectral properties

The effect of the molar concentration Rhodamine B dye on optical absorption spectra and fluorescence spectra. results show that Absorption intensity increased peaks are blue shifted as from 1 to 100 M. In addition, reveal an enhancement absorbance fluorescence quenching is observed. obtained higher than 60µM has come collisional mechanism. Beer-Lambert law governs at low concentrations. While deviates high concentrations due contributions dimers aggregation.